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KMID : 0376219700070020181
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Chonnam Medical Journal
1970 Volume.7 No. 2 p.181 ~ p.193
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Experimental study on the pathogenesis of splanic anemia
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Abstract
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Innormal and hypersplenic rabbits, the minute iron particles suspended in the gelatine solution were injected intravenously. The spleens of these animals were excised and diced into the small pieces and kept in the Trisma buffer containing trypsin and hyaluronidase. By means of the electromagnetic device the reticuloendothelial cells which had phagocyted the injected iron particles were separated from the lymphoid and other cells of the spleen, and these separated cells were suspended in the saline and homogenized.
In order to make a study on the inhibitory influences of spleen upon the blood corpuscles, the homogenate of reticuloendothelial cells (REC) and lymphoid and other cell(LOC)were injected respectively to two different animal peripheral blood cells were observed.
1. Erythrocyte. In both normal and splectomized animals, the injection counts whereas LOC(lymphoid and other cells) caused no significant changes of erythrocyte counts wheres LOC(lymphoid and other cells) caused slight decrease of erythrocytest in the splenectomized rabbitsy injection of LOC from the hypersplenic rabbits brought more marked decrease of eryhrocyte counts.
2. Leukocyte. In both normal and splenectomzied animals, the injection of REC had no significant effect on leukocyte counts. Injection of LOC caused general increase of leukocytes.
3. Reticulocyte and thrombocyte. The responses of reticulocytes and thrombocytes were somewhat similar to those of erythrocytes. In both normal and splenectomized animals, the injection of REC induced no significant changes of reticulocytes and thrombocytes, whereas LOC caused decrease of reticulocyte and thrombocyte count: in the splenectomized rabbits, LOC from the hypersplenic rabbits induced more marked decrease of reticulocyte and thrombocyte counts.
These experimental results suggest that the spleen may produce certain anemia inducing substance related to splenic anemia. It is also suggested that the orgin of such anemia inducing substance is not the reticuloendothelial cell but the lymphoid and other cells of the spleen.
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